5The same properties also contributed to Nazi Germany’s strategy against agar’s scarcity, which — besides being supplied from Japan by submarine — relied on large pre-war stocks and on recovery methods to reuse bacteriological agar by autoclaving (boiling at around 121°C, 250°F, in a pressurized container for 30 to 60 minutes), thus liquefying and sterilizing the jelly, before purifying it again.
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The original text on DIP from 1994.